Review



halomonas campaniensis ls21  (DSMZ)


Bioz Verified Symbol DSMZ is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    DSMZ halomonas campaniensis ls21
    Preliminary electroporation protocol of <t>Halomonas</t> elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).
    Halomonas Campaniensis Ls21, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halomonas campaniensis ls21/product/DSMZ
    Average 93 stars, based on 17 article reviews
    halomonas campaniensis ls21 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp."

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.70285

    Preliminary electroporation protocol of Halomonas elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).
    Figure Legend Snippet: Preliminary electroporation protocol of Halomonas elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).

    Techniques Used: Electroporation, Cell Culture, Plasmid Preparation, Incubation

    Preliminary electroporation tests of Halomonas elongata DSM 2581. (a) Electroporation efficiencies at different voltages (1.5, 1.7, 2.1 and 2.5 kV) and pulse numbers (2× 2.1, 2× 2.5 kV). (b) Electroporation efficiencies from electrocompetent cells prepared with sucrose (300 mM) vs. glycerol (10%). (c) Electroporation efficiencies from electrocompetent cells prepared from cells harvested at 6, 14 and 24 h. Data shown represent mean ± standard deviation from three biological replicates for panels (a) and (b), and four biological replicates for panel (c). Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; *** p < 0.001)
    Figure Legend Snippet: Preliminary electroporation tests of Halomonas elongata DSM 2581. (a) Electroporation efficiencies at different voltages (1.5, 1.7, 2.1 and 2.5 kV) and pulse numbers (2× 2.1, 2× 2.5 kV). (b) Electroporation efficiencies from electrocompetent cells prepared with sucrose (300 mM) vs. glycerol (10%). (c) Electroporation efficiencies from electrocompetent cells prepared from cells harvested at 6, 14 and 24 h. Data shown represent mean ± standard deviation from three biological replicates for panels (a) and (b), and four biological replicates for panel (c). Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; *** p < 0.001)

    Techniques Used: Electroporation, Standard Deviation, Negative Control, Plasmid Preparation

    Effect of salinity on electroporation efficiency and cell morphology of Halomonas elongata DSM 2581. (a) Electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 0.5%, 1%, 2% and 6%. Data shown represent geometric mean */ geometric SD factor from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (** p < 0.01; **** p < 0.0001). (b) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 1% NaCl. (c) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 6% NaCl. (d) Transmission electron microscopy imaging of cells cultured for 14 h in LB medium containing 6% NaCl.
    Figure Legend Snippet: Effect of salinity on electroporation efficiency and cell morphology of Halomonas elongata DSM 2581. (a) Electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 0.5%, 1%, 2% and 6%. Data shown represent geometric mean */ geometric SD factor from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (** p < 0.01; **** p < 0.0001). (b) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 1% NaCl. (c) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 6% NaCl. (d) Transmission electron microscopy imaging of cells cultured for 14 h in LB medium containing 6% NaCl.

    Techniques Used: Electroporation, Negative Control, Plasmid Preparation, Transmission Assay, Electron Microscopy, Imaging, Cell Culture

    Further optimisation of electroporation parameters for Halomonas elongata DSM 2581. (a) Comparison of electroporation efficiencies using two different volumes of electrocompetent cell suspension: 40 μL vs. 200 μL. (b) Effect of cell concentration on electroporation efficiency. Electrocompetent cells were resuspended in glycerol (10%) at different optical densities: OD 600 = 10, 20, 40, 60. (c) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under different electroporation conditions: Voltage, pulse number and resistance. (d) Table summarising the eight electroporation conditions tested in panel (c), resulting from the constrained mixed‐level orthogonal array design. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; * p < 0.05; *** p < 0.001; **** p < 0.0001).
    Figure Legend Snippet: Further optimisation of electroporation parameters for Halomonas elongata DSM 2581. (a) Comparison of electroporation efficiencies using two different volumes of electrocompetent cell suspension: 40 μL vs. 200 μL. (b) Effect of cell concentration on electroporation efficiency. Electrocompetent cells were resuspended in glycerol (10%) at different optical densities: OD 600 = 10, 20, 40, 60. (c) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under different electroporation conditions: Voltage, pulse number and resistance. (d) Table summarising the eight electroporation conditions tested in panel (c), resulting from the constrained mixed‐level orthogonal array design. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; * p < 0.05; *** p < 0.001; **** p < 0.0001).

    Techniques Used: Electroporation, Comparison, Suspension, Concentration Assay, Standard Deviation, Negative Control, Plasmid Preparation

    Effect of plasmid source on the electroporation efficiency of Halomonas elongata DSM 2581. (a) Electroporation efficiencies of pSEVA241 purified from either E. coli 10‐beta (NEB) or H. elongata DSM 2581. (b) Electroporation efficiencies of pSEVA231 purified from either E. coli 10‐beta (NEB), E. coli C2925 (NEB) or H. elongata DSM 2581. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241 (a) or pSEVA231 (b) (* p < 0.05; *** p < 0.001; **** p < 0.0001).
    Figure Legend Snippet: Effect of plasmid source on the electroporation efficiency of Halomonas elongata DSM 2581. (a) Electroporation efficiencies of pSEVA241 purified from either E. coli 10‐beta (NEB) or H. elongata DSM 2581. (b) Electroporation efficiencies of pSEVA231 purified from either E. coli 10‐beta (NEB), E. coli C2925 (NEB) or H. elongata DSM 2581. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241 (a) or pSEVA231 (b) (* p < 0.05; *** p < 0.001; **** p < 0.0001).

    Techniques Used: Plasmid Preparation, Electroporation, Purification, Standard Deviation, Negative Control

    Electroporation of Halomonas boliviensis LC1 and Halomonas campaniensis LS21. Electroporation efficiencies of H. boliviensis LC1 and H. campaniensis LS21 transformed with pSEVA231 purified from either E. coli C2925 (NEB) (a) Comparison of electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 6% vs. 1%. (b) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under varying electroporation conditions: Voltage, pulse number and resistance. Parameters for conditions C1, C4, C6 and C7 are detailed in Figure . Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA231. (*** p < 0.001; **** p < 0.0001)
    Figure Legend Snippet: Electroporation of Halomonas boliviensis LC1 and Halomonas campaniensis LS21. Electroporation efficiencies of H. boliviensis LC1 and H. campaniensis LS21 transformed with pSEVA231 purified from either E. coli C2925 (NEB) (a) Comparison of electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 6% vs. 1%. (b) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under varying electroporation conditions: Voltage, pulse number and resistance. Parameters for conditions C1, C4, C6 and C7 are detailed in Figure . Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA231. (*** p < 0.001; **** p < 0.0001)

    Techniques Used: Electroporation, Transformation Assay, Purification, Comparison, Standard Deviation, Negative Control, Plasmid Preparation



    Similar Products

    halomonas campaniensis ls21  (DSMZ)
    93
    DSMZ halomonas campaniensis ls21
    Preliminary electroporation protocol of <t>Halomonas</t> elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).
    Halomonas Campaniensis Ls21, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/halomonas campaniensis ls21/product/DSMZ
    Average 93 stars, based on 1 article reviews
    halomonas campaniensis ls21 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Preliminary electroporation protocol of Halomonas elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Preliminary electroporation protocol of Halomonas elongata DSM 2581. Cells were cultured in LB medium supplemented with NaCl (LB60) at 30°C and 225 rpm for 14 h. Electrocompetent cells were prepared by washing the harvested cells with CaCl 2 (50 mM) + NaCl (6%) for 5 min, followed by two sucrose (300 mM) washes. The cell pellet was then resuspended in sucrose and transferred to a 0.2‐cm gap electroporation cuvette, to which the plasmid DNA was subsequently added. Electroporation was performed using a Bio‐Rad MicroPulser, after which cells were recovered in LB60 supplemented with glucose (20 mM) at 30°C and 225 rpm for 90 min. Following recovery, electroporated cells were plated onto LB60 agar plates and incubated at 30°C for 36 h to allow for colony formation. (Created in BioRender. Rios Solis, L. (2025) https://BioRender.com/osb8xfd ).

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Electroporation, Cell Culture, Plasmid Preparation, Incubation

    Preliminary electroporation tests of Halomonas elongata DSM 2581. (a) Electroporation efficiencies at different voltages (1.5, 1.7, 2.1 and 2.5 kV) and pulse numbers (2× 2.1, 2× 2.5 kV). (b) Electroporation efficiencies from electrocompetent cells prepared with sucrose (300 mM) vs. glycerol (10%). (c) Electroporation efficiencies from electrocompetent cells prepared from cells harvested at 6, 14 and 24 h. Data shown represent mean ± standard deviation from three biological replicates for panels (a) and (b), and four biological replicates for panel (c). Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; *** p < 0.001)

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Preliminary electroporation tests of Halomonas elongata DSM 2581. (a) Electroporation efficiencies at different voltages (1.5, 1.7, 2.1 and 2.5 kV) and pulse numbers (2× 2.1, 2× 2.5 kV). (b) Electroporation efficiencies from electrocompetent cells prepared with sucrose (300 mM) vs. glycerol (10%). (c) Electroporation efficiencies from electrocompetent cells prepared from cells harvested at 6, 14 and 24 h. Data shown represent mean ± standard deviation from three biological replicates for panels (a) and (b), and four biological replicates for panel (c). Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; *** p < 0.001)

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Electroporation, Standard Deviation, Negative Control, Plasmid Preparation

    Effect of salinity on electroporation efficiency and cell morphology of Halomonas elongata DSM 2581. (a) Electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 0.5%, 1%, 2% and 6%. Data shown represent geometric mean */ geometric SD factor from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (** p < 0.01; **** p < 0.0001). (b) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 1% NaCl. (c) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 6% NaCl. (d) Transmission electron microscopy imaging of cells cultured for 14 h in LB medium containing 6% NaCl.

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Effect of salinity on electroporation efficiency and cell morphology of Halomonas elongata DSM 2581. (a) Electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 0.5%, 1%, 2% and 6%. Data shown represent geometric mean */ geometric SD factor from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (** p < 0.01; **** p < 0.0001). (b) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 1% NaCl. (c) Transmission electron microscopy imaging of cells cultured for 24 h in LB medium containing 6% NaCl. (d) Transmission electron microscopy imaging of cells cultured for 14 h in LB medium containing 6% NaCl.

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Electroporation, Negative Control, Plasmid Preparation, Transmission Assay, Electron Microscopy, Imaging, Cell Culture

    Further optimisation of electroporation parameters for Halomonas elongata DSM 2581. (a) Comparison of electroporation efficiencies using two different volumes of electrocompetent cell suspension: 40 μL vs. 200 μL. (b) Effect of cell concentration on electroporation efficiency. Electrocompetent cells were resuspended in glycerol (10%) at different optical densities: OD 600 = 10, 20, 40, 60. (c) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under different electroporation conditions: Voltage, pulse number and resistance. (d) Table summarising the eight electroporation conditions tested in panel (c), resulting from the constrained mixed‐level orthogonal array design. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; * p < 0.05; *** p < 0.001; **** p < 0.0001).

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Further optimisation of electroporation parameters for Halomonas elongata DSM 2581. (a) Comparison of electroporation efficiencies using two different volumes of electrocompetent cell suspension: 40 μL vs. 200 μL. (b) Effect of cell concentration on electroporation efficiency. Electrocompetent cells were resuspended in glycerol (10%) at different optical densities: OD 600 = 10, 20, 40, 60. (c) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under different electroporation conditions: Voltage, pulse number and resistance. (d) Table summarising the eight electroporation conditions tested in panel (c), resulting from the constrained mixed‐level orthogonal array design. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241. (ns, not significant; * p < 0.05; *** p < 0.001; **** p < 0.0001).

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Electroporation, Comparison, Suspension, Concentration Assay, Standard Deviation, Negative Control, Plasmid Preparation

    Effect of plasmid source on the electroporation efficiency of Halomonas elongata DSM 2581. (a) Electroporation efficiencies of pSEVA241 purified from either E. coli 10‐beta (NEB) or H. elongata DSM 2581. (b) Electroporation efficiencies of pSEVA231 purified from either E. coli 10‐beta (NEB), E. coli C2925 (NEB) or H. elongata DSM 2581. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241 (a) or pSEVA231 (b) (* p < 0.05; *** p < 0.001; **** p < 0.0001).

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Effect of plasmid source on the electroporation efficiency of Halomonas elongata DSM 2581. (a) Electroporation efficiencies of pSEVA241 purified from either E. coli 10‐beta (NEB) or H. elongata DSM 2581. (b) Electroporation efficiencies of pSEVA231 purified from either E. coli 10‐beta (NEB), E. coli C2925 (NEB) or H. elongata DSM 2581. Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA241 (a) or pSEVA231 (b) (* p < 0.05; *** p < 0.001; **** p < 0.0001).

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Plasmid Preparation, Electroporation, Purification, Standard Deviation, Negative Control

    Electroporation of Halomonas boliviensis LC1 and Halomonas campaniensis LS21. Electroporation efficiencies of H. boliviensis LC1 and H. campaniensis LS21 transformed with pSEVA231 purified from either E. coli C2925 (NEB) (a) Comparison of electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 6% vs. 1%. (b) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under varying electroporation conditions: Voltage, pulse number and resistance. Parameters for conditions C1, C4, C6 and C7 are detailed in Figure . Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA231. (*** p < 0.001; **** p < 0.0001)

    Journal: Microbial Biotechnology

    Article Title: Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

    doi: 10.1111/1751-7915.70285

    Figure Lengend Snippet: Electroporation of Halomonas boliviensis LC1 and Halomonas campaniensis LS21. Electroporation efficiencies of H. boliviensis LC1 and H. campaniensis LS21 transformed with pSEVA231 purified from either E. coli C2925 (NEB) (a) Comparison of electroporation efficiencies from electrocompetent cells prepared from cultures grown in LB medium containing different concentrations of NaCl: 6% vs. 1%. (b) Comparison of electroporation efficiencies using two different electroporator systems—Bio‐Rad MicroPulser vs. Bio‐Rad Gene Pulser—under varying electroporation conditions: Voltage, pulse number and resistance. Parameters for conditions C1, C4, C6 and C7 are detailed in Figure . Data shown represent mean ± standard deviation from three biological replicates. Negative control experiments were performed by electroporating electrocompetent cells without the addition of plasmid pSEVA231. (*** p < 0.001; **** p < 0.0001)

    Article Snippet: Halomonas elongata DSM 2581, Halomonas boliviensis LC1 and Halomonas campaniensis LS21 were obtained from DSMZ.

    Techniques: Electroporation, Transformation Assay, Purification, Comparison, Standard Deviation, Negative Control, Plasmid Preparation